Acupuncture is used to control various emotional disorders. HT7 stimulation has been found to improve insomnia and depression, and to modulate inflammatory cytokines. The study's objective was to evaluate the potential of HT7 for modulating the inflammatory response in the medial septum (MS) region in rats in which insomnia was induced by administration of caffeine for 2 weeks. Rats were randomly assigned to 4 groups: control (DW), caffeine (Caff), caffeine + acupuncture (HT7), and caffeine + nonacupuncture (NA). Wake, REM, and nREM sleep durations were evaluated using a rat wireless EEG measurement device. The behavioral patterns of rats were measured through an open field test. Anti- and pro-inflammatory cytokine levels were measured in the MS region using a cytokine array. The levels of M2 microglia (arginase-1) in the MS region were confirmed through immunofluorescence staining. IL-10 and IL-4 levels were measured by ELISA. Finally, the expression levels of pSTAT6/STAT6 and p-P38/P38 were estimated by Western blot assay. HT7 stimulation restored the arousal time increased by caffeine. HT7 stimulation increased M2 microglia expression and increased anti-inflammatory cytokine levels in the MS region. In addition, increased expression of lL-4 regulated the phosphorylation of STAT6 and P38, which are members of sub signaling pathways. HT7 stimulation may improve sleep patterns by regulating the cholinergic anti-inflammatory pathway and increasing the expression of anti inflammatory markers.