Persistent inflammation contributes to the pathogenesis of various diseases, including cardiovascular disease, rheumatologic disorders, and multiple organ failure. To study the mechanisms of inflammation and develop therapeutic agents, researchers have created extensive experimental models. Recently, zebrafish has become a valuable model for researching inflammatory mechanisms in vivo, due to its genetic similarity to humans and the availability of diverse genetic manipulation techniques. Moreover, the small size, rapid embryonic development, and high fecundity of zebrafish make it advantageous for pharmacological screening. Researchers have created zebrafish models to induce inflammation, screening natural products or traditional herbal medicines with anti-inflammatory properties. Methods such as lipopolysaccharide (LPS) immersion or tail-cutting have been used to observe inflammation through the distribution of neutrophils, which serve as a first line of defense and can contribute to wound healing or persistent inflammation. By using natural products and herbal medicines, such as bee venom and deer antler velvet, we performed screening to discover immunosuppressive candidates. Through LPS immersion in zebrafish models, we identified and verified several candidates with immunosuppressive functions. Our screening platform using the zebrafish inflammation model will provide a highly efficient method for developing new anti-inflammatory agents.